Tier 3 — preclinical

Betaine promotes cell differentiation of human osteoblasts in primary culture

Isabella Villa, Pamela Senesi, Anna Montesano, Anita Ferraretto, Fernanda Vacante, Alice Spinello, Michela Bottani, Simona Bolamperti, Alessandro Rubinacci, Livio Luzi, Ileana Terruzzi
Journal of translational medicine 2017 15(1):132

Bibliography

PubMed
PMID 28592272
PubMed Central
PMC5463390
DOI
10.1186/s12967-017-1233-5
Funding
Funding was not specified in the indexed record or accessible funding metadata.
Competing interests
Reported disclosure: Competing interestsAll authors declare that they have no competing interests.

Study snapshot

DesignExperimental animal and/or laboratory research
ModelPrimary human osteoblast cultures
SampleExperiment-specific biological replicates and model groups; see original methods
InterventionBetaine 10 mmol/L
DurationMinutes through 24 hours
EndpointsOsteoblast differentiation; Calcium signaling

What the study showed, in plain terms

Published in 2017, this source examined preclinical aging, brain and metabolic mechanisms. Design: Experimental animal and/or laboratory research. Sample/model: Experiment-specific biological replicates and model groups; see original methods. The exposure or intervention was Betaine 10 mmol/L. Follow-up or treatment period: Minutes through 24 hours.

Betaine increased selected osteogenic genes and calcium, ERK and IGF-related signaling in cultured bone cells.

Experimental model evidence. The intervention, concentrations and outcomes do not establish an effective human supplement dose or a clinical treatment benefit.

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Key findings

  • Betaine increased selected osteogenic genes and calcium, ERK and IGF-related signaling in cultured bone cells.
  • The recorded outcome domains were Osteoblast differentiation; Calcium signaling.
  • The result applies to the tested exposure: Betaine 10 mmol/L; Minutes through 24 hours.

What this study can and cannot tell us

  • Experimental model evidence. The intervention, concentrations and outcomes do not establish an effective human supplement dose or a clinical treatment benefit.
  • Cell concentrations, species, disease models and experimental routes cannot be converted directly into an effective or safe oral human regimen.
  • Source basis: Accessible full-text extract plus indexed bibliographic data. Missing method details, funding information or participant denominators are not inferred.

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Editorial review

Reviewed by the Biohack Blueprint research team

Last verified

Dr. Khaled Mahmoud, MBBChCritical Care Medicine, Longevity Science & Editorial Direction Dr. Amr Yasser, PhDCardiometabolic, Musculoskeletal, Gut & Bioavailability Dr. Abdulla Mostafa, PhDCellular Senescence, Neurological, Mitochondrial & Brain-Ageing Biology