Tier 1 — highest

Co-administration of vitamin D and N-acetylcysteine to modulate immunosenescence in older adults with vitamin D deficiency: a randomized clinical trial

Samira Rastgoo, Katayoun Pourvali, Seyed Ahmad Raeissadat, Ghazaleh Eslamian, Hamid Zand
Frontiers in immunology 2025 16:1570441

Bibliography

PubMed
PMID 40421021
PubMed Central
PMC12104082
DOI
10.3389/fimmu.2025.1570441
Funding
Support is recorded from Shahid Beheshti University of Medical Sciences. See the original publication for grant and material-support details.
Competing interests
The publication includes author disclosures; consult the linked source for the individual relationships and their relevance.

Study snapshot

DesignRandomized controlled clinical trial
ModelHuman participants
Sample88 vitamin D-deficient older adults; 22 per group
InterventionVitamin D 1,000 or 5,000 IU/day, with or without NAC 600 mg/day
Duration8 weeks
EndpointsPBMC senescence markers; Senescence-related gene expression; Inflammatory markers

What the study showed, in plain terms

This publication examines pbmc senescence markers, senescence-related gene expression, inflammatory markers. Study population or evidence base: 88 vitamin D-deficient older adults; 22 per group. Intervention or exposure: Vitamin D 1,000 or 5,000 IU/day, with or without NAC 600 mg/day.

Key findings

The higher vitamin D dose, with or without NAC, improved selected immune-cell senescence markers compared with the lower-dose arm. Circulating inflammation measures were not significantly different.

What this study can and cannot tell us

Short biomarker study without a vitamin-D-free placebo arm. It does not demonstrate whole-body reversal of aging or long-term safety of the higher dose.

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