Tier 3 — preclinical

Quantitative Analysis of NAD Synthesis-Breakdown Fluxes

Liu L, Su X, Quinn WJ 3rd, Hui S, Krukenberg K, Frederick DW, Redpath P, Zhan L, Chellappa K, White E, Migaud M, Mitchison TJ, Baur JA, Rabinowitz JD
Cell metabolism 2018 27; 5; 1067-1080.e5

Bibliography

PubMed
PMID 29685734
PubMed Central
PMC5932087
DOI
10.1016/j.cmet.2018.03.018
Funding
Indexed grant support includes NIDDK NIH HHS, NCI NIH HHS, NIGMS NIH HHS, Biotechnology and Biological Sciences Research Council, NIA NIH HHS. The complete funding declaration is not specified in the accessible abstract.
Competing interests
A complete competing-interest declaration is not specified in the accessible publication information.

Study snapshot

DesignPreclinical animal study with supporting assays as applicable
ModelCells and mice
SampleIsotope-tracing experiments across cells and mouse tissues
InterventionLabeled precursors given orally or intravenously
DurationAcute and tissue-specific tracer time courses
EndpointsModel-specific physiological outcomes; Biochemical or functional measures reported in the study

What the study showed, in plain terms

NAD+ synthesis and breakdown varied by tissue, and oral versus intravenous precursor delivery produced different metabolic handling.

Key findings

NAD+ synthesis and breakdown varied by tissue, and oral versus intravenous precursor delivery produced different metabolic handling.

What this study can and cannot tell us

Tracer results in cells and mice cannot be used as a direct human oral-versus-infusion efficacy comparison.

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Editorial review

Reviewed by the Biohack Blueprint research team

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Dr. Khaled Mahmoud, MBBChCritical Care Medicine, Longevity Science & Editorial Direction Dr. Amr Yasser, PhDCardiometabolic, Musculoskeletal, Gut & Bioavailability Dr. Abdulla Mostafa, PhDCellular Senescence, Neurological, Mitochondrial & Brain-Ageing Biology