Tier 4 — mechanistic

NRK1 controls nicotinamide mononucleotide and nicotinamide riboside metabolism in mammalian cells

Ratajczak J, Joffraud M, Trammell SA, Ras R, Canela N, Boutant M, Kulkarni SS, Rodrigues M, Redpath P, Migaud ME, Auwerx J, Yanes O, Brenner C, Cantó C
Nature communications 2016 7; 13103

Bibliography

PubMed
PMID 27725675
PubMed Central
PMC5476803
DOI
10.1038/ncomms13103
Funding
Indexed grant support includes NHLBI NIH HHS, NIAAA NIH HHS, NIGMS NIH HHS. The complete funding declaration is not specified in the accessible abstract.
Competing interests
Disclosures include Nestlé employment and ChromaDex-related equity, research, consulting and advisory relationships, plus supplement-business interests.

Study snapshot

DesignIn vitro or biochemical mechanistic study
ModelMammalian cells and experimental genetic models
SampleGenetic gain/loss and stable-isotope experiments
InterventionNR, NMN and other precursors in NRK1 pathway experiments
DurationAssay-specific experimental periods
EndpointsNAD+ pathway or cellular-function measures

What the study showed, in plain terms

The study identified NRK1-dependent handling of NR and extracellular NMN in the tested mammalian models.

Key findings

The study identified NRK1-dependent handling of NR and extracellular NMN in the tested mammalian models.

What this study can and cannot tell us

Cellular uptake and enzymatic results are context-dependent and do not establish clinical superiority or equivalence of oral precursors.

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Belenky P et al. · Cell · 2007
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Discoveries of nicotinamide riboside as a nutrient and conserved NRK genes establish a Preiss-Handler independent route to NAD+ in fungi and humans
Bieganowski P et al. · Cell · 2004

Editorial review

Reviewed by the Biohack Blueprint research team

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Dr. Khaled Mahmoud, MBBChCritical Care Medicine, Longevity Science & Editorial Direction Dr. Amr Yasser, PhDCardiometabolic, Musculoskeletal, Gut & Bioavailability Dr. Abdulla Mostafa, PhDCellular Senescence, Neurological, Mitochondrial & Brain-Ageing Biology