Tier 4 — mechanistic

Double-Pronged NAD Preservation: Delaying Cellular Senescence and Initiating Musculoskeletal Regeneration

Jianfeng Yu, Mingzhuang Hou, Yaoge Deng, Chenqi Yu, Yang Liu, Kang Kang, Xiaowei Xia, Xiaoping Li, Huilin Yang, Dinghua Jiang, Wu Xu, Yijian Zhang, Xuesong Zhu
Aging Cell 2026 25(4):e70468

Bibliography

PubMed
PMID 41944220
PubMed Central
PMC13054677
DOI
10.1111/acel.70468

Study snapshot

DesignPreclinical combination-intervention study
ModelSkeletal precursor cells and aged mice
Sample
InterventionNMN plus apigenin combination ('N + A' regimen)
Duration
EndpointsCellular senescence; Cartilage degeneration; Bone loss; Muscle atrophy; Exercise capacity; NAD+ and SIRT3 signaling

What the study showed, in plain terms

This 2026 study combined NMN with apigenin to preserve NAD+ and test musculoskeletal regeneration in preclinical models.

Key findings

The combination reduced senescence markers, improved cartilage, bone and muscle outcomes in aged mice and enhanced exercise capacity, with SIRT3-related mitochondrial signaling implicated.

What this study can and cannot tell us

This was a combination intervention in preclinical models. The effects cannot be assigned to apigenin alone, and there is no human efficacy evidence from this study.

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Editorial review

Reviewed by the Biohack Blueprint research team

Last verified

Dr. Khaled Mahmoud, MBBChCritical Care Medicine, Longevity Science & Editorial Direction Dr. Amr Yasser, PhDCardiometabolic, Musculoskeletal, Gut & Bioavailability Dr. Abdulla Mostafa, PhDCellular Senescence, Neurological, Mitochondrial & Brain-Ageing Biology