Tier 2 — strong

Betaine-, xylitol-, and extra virgin olive oil-based toothpaste modulates inflammation and oral nitric oxide pathway: Clinical trial in gingivitis

Francisco Mesa, Alejandro Rodríguez-Agurto, Marco Bonilla, Debora Rodríguez-Vilaboa, Beatriz Rodríguez-Vilaboa, Manuel Bravo
Journal of periodontology 2026 :10.1002/jper.70135

Bibliography

PubMed
PMID 41981946
DOI
10.1002/jper.70135
Funding
Indexed grant support: University of Granada and Mucosal Innovations SL under the research Project. The indexed list may not contain the complete funding declaration.
Competing interests
A competing-interest declaration was not available in the indexed record or accessible disclosure metadata.

Study snapshot

DesignRandomized controlled trial
ModelHuman participants
Sample96 patients with gingivitis completing treatment
InterventionOlive oil, xylitol and betaine toothpaste versus placebo or commercial toothpaste
Duration4 months
EndpointsGingival elastase; Salivary nitrate and nitrite

What the study showed, in plain terms

Published in 2026, this source examined betaine hcl and topical formulations. Design: Randomized controlled trial. Sample/model: 96 patients with gingivitis completing treatment. The exposure or intervention was Olive oil, xylitol and betaine toothpaste versus placebo or commercial toothpaste. Follow-up or treatment period: 4 months.

The test formulation improved the salivary nitrite-to-nitrate ratio. The elastase comparison with commercial toothpaste was borderline at P=0.050.

Combination product and local biomarkers; no isolated TMG effect or general cardiovascular nitric-oxide benefit was tested.

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Continue with Betaine Anhydrous vs Betaine HCl: Which Form Is TMG?

Key findings

  • The test formulation improved the salivary nitrite-to-nitrate ratio.
  • The elastase comparison with commercial toothpaste was borderline at P=0.050.
  • The recorded outcome domains were Gingival elastase; Salivary nitrate and nitrite.
  • The result applies to the tested exposure: Olive oil, xylitol and betaine toothpaste versus placebo or commercial toothpaste; 4 months.

What this study can and cannot tell us

  • Combination product and local biomarkers; no isolated TMG effect or general cardiovascular nitric-oxide benefit was tested.
  • An individual controlled trial must be interpreted alongside its comparator, sample size, endpoint selection and independent replication. It does not validate a different commercial formulation or a general longevity claim.
  • Source basis: Indexed abstract and bibliographic data. Missing method details, funding information or participant denominators are not inferred.

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Editorial review

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Dr. Khaled Mahmoud, MBBChCritical Care Medicine, Longevity Science & Editorial Direction Dr. Amr Yasser, PhDCardiometabolic, Musculoskeletal, Gut & Bioavailability Dr. Abdulla Mostafa, PhDCellular Senescence, Neurological, Mitochondrial & Brain-Ageing Biology