Tier 3 — preclinical

Impact of Methyl-Donor Micronutrient Supplementation on DNA Methylation Patterns: A Systematic Review and Meta-Analysis of in vitro, Animal, and Human Studies

Jhulia Caroline N L da Mota, Amanda A Ribeiro, Lucas M Carvalho, Gabriel P Esteves, Sofia M Sieczkowska, Karla F Goessler, Bruno Gualano, Carolina F Nicoletti
Lifestyle genomics 2023 16(1):192-213

Bibliography

PubMed
PMID 37935134
DOI
10.1159/000533193
Funding
Funding was not specified in the indexed record or accessible funding metadata.
Competing interests
A competing-interest declaration was not available in the indexed record or accessible disclosure metadata.

Study snapshot

DesignSystematic review with mixed-model meta-analyses
ModelHuman, animal and in vitro evidence synthesis
Sample57 studies: 18 human, 35 animal and 4 in vitro
InterventionMethyl-donor nutrients; pooled analyses mainly concerned folic acid
DurationVaried protocols across the literature
EndpointsDNA methylation; Methyl-donor nutrients

What the study showed, in plain terms

Published in 2023, this source examined safety assessments and evidence synthesis. Design: Systematic review with mixed-model meta-analyses. Sample/model: 57 studies: 18 human, 35 animal and 4 in vitro. The exposure or intervention was Methyl-donor nutrients; pooled analyses mainly concerned folic acid. Follow-up or treatment period: Varied protocols across the literature.

Animal folate studies suggested dose-related methylation changes, but pooled human folic-acid trials did not show a significant global DNA-methylation effect.

Mixed nutrients and models, with many bias concerns. This is not proof that TMG reverses epigenetic age or prevents overmethylation.

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Key findings

  • Animal folate studies suggested dose-related methylation changes, but pooled human folic-acid trials did not show a significant global DNA-methylation effect.
  • The recorded outcome domains were DNA methylation; Methyl-donor nutrients.
  • The result applies to the tested exposure: Methyl-donor nutrients; pooled analyses mainly concerned folic acid; Varied protocols across the literature.

What this study can and cannot tell us

  • Mixed nutrients and models, with many bias concerns. This is not proof that TMG reverses epigenetic age or prevents overmethylation.
  • Cell concentrations, species, disease models and experimental routes cannot be converted directly into an effective or safe oral human regimen.
  • Source basis: Indexed abstract and bibliographic data. Missing method details, funding information or participant denominators are not inferred.

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Editorial review

Reviewed by the Biohack Blueprint research team

Last verified

Dr. Khaled Mahmoud, MBBChCritical Care Medicine, Longevity Science & Editorial Direction Dr. Amr Yasser, PhDCardiometabolic, Musculoskeletal, Gut & Bioavailability Dr. Abdulla Mostafa, PhDCellular Senescence, Neurological, Mitochondrial & Brain-Ageing Biology