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Exogenous myrosinase from mustard seed increases bioavailability of sulforaphane from a glucoraphanin-rich broccoli seed extract in a randomized clinical study
Scientific Reports
2026
16(1)
Bibliography
- PubMed
- PMID 41692762
- PubMed Central
- PMC12996352
- Funding
- NSF DGE-1922560 and National Institute of Food and Agriculture grants ME022303 and ME022329.
- Competing interests
- Angela Mastaloudis was employed by Brassica Protection Products, LLC; Jed W Fahey was a consultant to Brassica Protection Products, LLC. Other authors declared no conflicts.
Study snapshot
| Design | Randomized, double-blind crossover clinical study |
|---|---|
| Model | Healthy adults |
| Sample | 16 participants (9 female, 7 male) |
| Intervention | Single oral dose of glucoraphanin-rich broccoli seed extract with myrosinase-containing mustard seed powder versus broccoli seed extract alone; both conditions included ascorbic acid. |
| Duration | |
| Endpoints | Sulforaphane bioavailability; Urinary sulforaphane metabolites; Glucoraphanin conversion rate; Gut microbial glucoraphanin-converting genes; Fecal bacterial communities |
What the study showed, in plain terms
Key findings
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