Tier 4 — mechanistic

Control analysis of collagen synthesis by glycine, proline and lysine in bovine chondrocytes in vitro - Its relevance for medicine and nutrition

de Paz-Lugo P, Lupiáñez JA, Sicilia J, Meléndez-Hevia E
Bio Systems 2023 232:105004

Bibliography

PubMed
PMID 37598999
DOI
10.1016/j.biosystems.2023.105004
Funding
Funding was not specified in the indexed abstract; the full funding disclosure has not been verified.
Competing interests
The accessible abstract does not establish the authors' competing interests; absence of a statement is not evidence of no conflicts.

Study snapshot

DesignLaboratory mechanistic study
ModelBovine chondrocytes in vitro
SampleBovine chondrocyte cultures; independent culture counts not specified in the indexed abstract
InterventionManipulation of glycine, proline and lysine availability; metabolic-control analysis
DurationIn-vitro experiments
EndpointsCollagen-synthesis flux; Intracellular procollagen degradation

What the study showed, in plain terms

Cultured bovine cartilage cells showed amino-acid-dependent control of collagen synthesis. The authors proposed that glycine supply could influence inefficient procollagen production.

Key findings

  • Glycine was a major influence in the reported metabolic-control analysis.
  • Proline and lysine availability also contributed.

What this study can and cannot tell us

Cell-culture substrate availability does not establish dietary deficiency in people.

No human osteoarthritis, skin or joint-outcome trial was conducted.

Related papers in the corpus

Tier 2
Collagen peptide supplementation before bedtime reduces sleep fragmentation and improves cognitive function in physically active males with sleep complaints
Thomas C et al. · European journal of nutrition · 2024
Tier 2
Coingestion of Collagen With Whey Protein Prevents Postexercise Decline in Plasma Glycine Availability in Recreationally Active Men
Aussieker T et al. · International journal of sport nutrition and exercise metabolism · 2024
Tier 4
mTORC1 amplifies the ATF4-dependent de novo serine-glycine pathway to supply glycine during TGF-β 1 -induced collagen biosynthesis
Selvarajah B et al. · Science signaling · 2019
Tier 4
A weak link in metabolism: the metabolic capacity for glycine biosynthesis does not satisfy the need for collagen synthesis
Meléndez-Hevia E et al. · Journal of biosciences · 2009
Tier 3
Glycine induces MafG to regulate glutathione metabolism, inhibit chondrocyte ferroptosis, and upregulate plectin to improve osteoarthritis
Li Y et al. · Cellular signalling · 2026
Tier 2
The effect of glycine administration on the characteristics of physiological systems in human adults: A systematic review
Soh J et al. · GeroScience · 2024

Editorial review

Reviewed by the Biohack Blueprint research team

Last verified

Dr. Khaled Mahmoud, MBBChCritical Care Medicine, Longevity Science & Editorial Direction