Tier 4 — mechanistic

Evaluation of apigenin using in vitro cytochalasin blocked micronucleus assay

Sanjeev Noel, Madhu Kasinathan, Srikanta Kumar Rath
Toxicology in Vitro 2006 20(7):1168-1172

Bibliography

PubMed
PMID 16759833
DOI
10.1016/j.tiv.2006.03.007
Funding
Not reported in the indexed record reviewed.
Competing interests
Not reported in the indexed record reviewed.

Study snapshot

DesignIn vitro cytochalasin-B blocked micronucleus assay
ModelCultured human peripheral blood lymphocytes
SampleCell-culture assay; donor/sample count was not stated in the PubMed abstract.
InterventionApigenin at 10, 33 and 100 micromolar in 5 mL lymphocyte cultures
DurationIn vitro exposure during micronucleus assay
EndpointsMicronucleus frequency; Dose-response for chromosomal damage signal

What the study showed, in plain terms

This laboratory study tested whether relatively high concentrations of apigenin could induce micronuclei, a marker used to screen for chromosomal damage, in cultured human lymphocytes.

Key findings

Apigenin increased micronucleus formation in a dose-dependent manner across the tested in vitro concentrations, identifying a potential genotoxic hazard signal under these laboratory conditions.

What this study can and cannot tell us

This was an in vitro assay at micromolar concentrations, not an oral human exposure study. It does not show that customary dietary or supplement doses cause chromosomal damage in people, and actual tissue exposure after oral dosing may differ substantially.

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Dr. Khaled Mahmoud, MBBChCritical Care Medicine, Longevity Science & Editorial Direction Dr. Amr Yasser, PhDCardiometabolic, Musculoskeletal, Gut & Bioavailability Dr. Abdulla Mostafa, PhDCellular Senescence, Neurological, Mitochondrial & Brain-Ageing Biology