Tier 3 — preclinical

High-Intensity Interval Training Combined with Glycine Supplementation Drives Ferroptosis Resistance to Counteract Skeletal Muscle Atrophy in Aging Mice

Ni PS, Jia MM, He JH, Yu Z, Li FH
Medicine and science in sports and exercise 2025 57(9):1852-1865

Bibliography

PubMed
PMID 40197604
DOI
10.1249/mss.0000000000003722
Funding
Funding was not specified in the indexed abstract; the full funding disclosure has not been verified.
Competing interests
The accessible abstract does not establish the authors' competing interests; absence of a statement is not evidence of no conflicts.

Study snapshot

DesignControlled animal intervention with mechanistic experiments
ModelAged male C57BL/6J mice
Sample16 male C57BL/6J mice aged 19 months; four groups
InterventionGlycine supplementation with or without high-intensity interval training
Duration8 weeks
EndpointsGrip strength; Muscle-fiber area; Ferroptosis and senescence markers

What the study showed, in plain terms

An aged-mouse experiment reported muscle benefits from combining glycine with interval training and explored ferroptosis-related mechanisms. It is not a human exercise recommendation.

Key findings

  • The combined treatment improved selected strength and muscle-fiber outcomes.
  • Transcriptomic and laboratory findings implicated ferroptosis-related pathways.

What this study can and cannot tell us

Four animals per group and multiple mechanistic outcomes create substantial uncertainty.

Docking predictions are not proof of a functional drug target or human clinical efficacy.

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Editorial review

Reviewed by the Biohack Blueprint research team

Last verified

Dr. Khaled Mahmoud, MBBChCritical Care Medicine, Longevity Science & Editorial Direction