Tier 3 — preclinical

Human Supplementation with AM3, Spermidine, and Hesperidin Enhances Immune Function, Decreases Biological Age, and Improves Oxidative-Inflammatory State: A Randomized Controlled Trial

Judith Félix, Estefanía Díaz-Del Cerro, Adriana Baca, Ana López-Ballesteros, María José Gómez-Sánchez, Mónica De la Fuente

Antioxidants (Basel) 2024 13(11):1391

Bibliography

PubMed: PMID 39594533
PubMed Central: PMC11591374
DOI: 10.3390/antiox13111391
Funding: Funded by Industrial Farmacéutica Cantabria, S.A.
Competing interests: The authors declared no conflicts of interest. The study sponsor was Industrial Farmacéutica Cantabria, S.A.

Study snapshot

Design	Randomized placebo-controlled trial of a multi-ingredient supplement
Model	Adults aged 30-63 years
Sample	41
Intervention	Two daily capsules providing AM3 150 mg, spermidine 0.6 mg and hesperidin 50 mg versus placebo
Duration	2 months
Endpoints	Immunity Clock biological age; Leukocyte immune function; Redox markers; Inflammatory cytokines

What the study showed, in plain terms

A small randomized trial reported favorable immune, redox and inflammation changes after a two-month supplement containing AM3, 0.6 mg spermidine and hesperidin. The study also reported a large change in an Immunity Clock estimate of biological age.

Key findings

The trial is relevant because spermidine was part of a human oral intervention, but it does not show that spermidine alone caused the immune or biological-age findings.

What this study can and cannot tell us

Small sample, short duration, three-active-ingredient formulation, surrogate immune/redox endpoints and an experimental biological-age model. Product attribution to spermidine is not possible.

Editorial review

Reviewed by the Biohack Blueprint research team

Khaled Mahmoud