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Increased Nitrate Intake From Beetroot Juice Over 4 Weeks Changes the Composition of the Oral, But Not the Intestinal Microbiome

Rebeka Fejes, Joana Séneca, Petra Pjevac, Martin Lutnik, Stefan Weisshaar, Nina Pilat, Romy Steiner, Karl-Heinz Wagner, Richard J Woodman, Catherine P Bondonno, Jonathan M Hodgson, David Berry, Michael Wolzt, Oliver Neubauer
Molecular Nutrition & Food Research 2025 69(20):e70156

Bibliography

PubMed
PMID 40522148
PubMed Central
PMC12538535
Funding
Austrian Science Fund (FWF) Clinical Research (KLIF), project KLI 858 awarded to Oliver Neubauer; open-access funding through University of Vienna/KEMO.
Competing interests
Authors declare no conflicts of interest (Wiley article).

Study snapshot

DesignRandomized double-blind placebo-controlled crossover trial
Model15 adults aged 56–71 with treated hypertension; clinical cohort overlaps Fejes 2024
Sample15 older adults with treated hypertension; overlaps Fejes 2024 clinical cohort
InterventionFour weeks nitrate-rich versus nitrate-depleted beetroot juice
DurationFour weeks of nitrate-rich versus nitrate-depleted beetroot juice per crossover condition
EndpointsOral and intestinal microbiome composition

What the study showed, in plain terms

Nitrate-rich juice increased oral Neisseria and decreased Veillonella relative abundance, without significant intestinal microbiome changes.

Key findings

Nitrate-rich juice increased oral Neisseria and decreased Veillonella relative abundance, without significant intestinal microbiome changes.

What this study can and cannot tell us

Overlapping participants with Fejes 2024 means these are separate publications, not independent clinical cohorts; microbiome shifts are surrogate outcomes.

Editorial review

Reviewed by the Biohack Blueprint research team

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