Tier 2 — strong
Ageing modifies the oral microbiome, nitric oxide bioavailability and vascular responses to dietary nitrate supplementation
Free Radical Biology and Medicine
2025
238:682–696
Bibliography
- PubMed
- PMID 40615058
- Funding
- BBSRC Industrial Partnership Award with DuPont Nutrition Biosciences Aps (BB/P022162/1; now International Flavors & Fragrances, IFF); publisher also acknowledges MRC/Wellcome/BBSRC core-facility support.
- Competing interests
- The authors formally declare no conflicts; the paper reports an industrial partnership award involving DuPont Nutrition Biosciences (now IFF) and thanks three IFF Health personnel for critical review of the manuscript. Both facts should accompany any appraisal.
Study snapshot
| Design | Randomized double-blind placebo-controlled crossover trial |
|---|---|
| Model | 39 younger adults (18–30 years) and 36 older adults (67–79 years) |
| Sample | 75 participants total (39 younger; 36 older) |
| Intervention | Two-week nitrate-rich beetroot juice, placebo juice, and antiseptic mouthwash conditions separated by washouts |
| Duration | Three two-week interventions with two-week washouts |
| Endpoints | Oral microbiome composition; Plasma nitrite; Blood pressure; Flow-mediated dilation |
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- Mouthwash, Oral Bacteria and Nitric Oxide: What Research ShowsDoes mouthwash reduce nitric oxide? Examine oral nitrate-reducing bacteria, small human trials, blood-pressure signals and how to protect dental health.Read analysis
- Nitric Oxide and Aging: How Vascular Signaling ChangesLearn how nitric oxide signaling can change with aging, the roles of endothelial function and oral bacteria, and what clinical trials have...Read analysis
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