Tier 2 — strong

The Effects of Short-Term N-Acetylcysteine Supplementation on Biochemical Parameters in Endurance-Trained Adults: A Randomized Clinical Trial

Marcin Sadowski, Emilia Zawieja, Agata Muzsik-Kazimierska, Ewa Bulczak, Agata Chmurzynska
Metabolites 2026 16(7):505

Bibliography

PubMed
PMID 42506456
PubMed Central
PMC13414332
DOI
10.3390/metabo16070505
Funding
Open-access publication costs were financed by the Polish Minister of Science and Higher Education through the Poznan University of Life Sciences research strategy for 2024–2026.
Competing interests
The authors declared no conflicts of interest.

Study snapshot

DesignRandomized double-blind placebo-controlled crossover trial
ModelHuman
Sample77 endurance-trained adults: 56 men and 21 women
InterventionOral NAC 1,200 mg/day
Duration7 days
EndpointsGlutathione; Homocysteine; Lipids; Liver enzymes; Biochemical safety markers

What the study showed, in plain terms

Large modern crossover trial evaluating short-term NAC effects on biochemical markers in endurance-trained adults.

Key findings

Provides 2026 sex-inclusive data on short-term redox and metabolic responses to 1,200 mg/day NAC.

What this study can and cannot tell us

Biochemical endpoints predominate; seven days does not establish long-term performance or adaptation effects.

Editorial review

Reviewed by the Biohack Blueprint research team

Last verified

Dr. Khaled Mahmoud, MBBChCritical Care Medicine, Longevity Science & Editorial Direction Dr. Amr Yasser, PhDCardiometabolic, Musculoskeletal, Gut & Bioavailability Dr. Abdulla Mostafa, PhDCellular Senescence, Neurological, Mitochondrial & Brain-Ageing Biology