Tier 2 — strong

Validity and reliability of test strips for the measurement of salivary nitrite concentration with and without the use of mouthwash in healthy adults

Abrar M Babateen, Oliver M Shannon, John C Mathers, Mario Siervo
Nitric Oxide 2019 91:15–22

Bibliography

PubMed
PMID 31295558
DOI
10.1016/j.niox.2019.07.002
Funding
Not yet independently verified in full text
Competing interests
Not yet independently verified in full text

Study snapshot

DesignRandomized crossover validation study
ModelHealthy adults
Sample10 participants
InterventionNitrate-rich beetroot juice after water or antibacterial-mouthwash rinse; salivary strip readings compared with lab assays
DurationAcute sampling through five hours after beetroot juice
EndpointsSalivary nitrite; Strip inter-rater and intra-rater reliability; Agreement with Griess and chemiluminescence assays

What the study showed, in plain terms

Salivary nitrite test strips tracked changes in salivary nitrite reasonably, but did not accurately quantify absolute nitrite values compared with laboratory methods.

Key findings

Strong correlation with laboratory assays for trends but poor Bland–Altman agreement for absolute concentrations. These strips are not direct measurements of circulating nitric oxide.

What this study can and cannot tell us

Ten healthy volunteers, acute protocol and one tested strip platform. Important method caveat when interpreting Nitralis salivary-strip findings.

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Editorial review

Reviewed by the Biohack Blueprint research team

Last verified

Dr. Khaled Mahmoud, MBBChCritical Care Medicine, Longevity Science & Editorial Direction